nuffnang

Thursday, November 18, 2010

Life in tissue culture lab 1

once more i face a long and arduous day just doing procedures for my experiment. this week is the MTT assay which is actually a simple process, but being my experiment which needs 6 plants, this simple process is lengthened considerably, which different concentrations and 96 wells to fill. to put this part of my research in a bottle, it is to
1) culture cells, count them, calculate how much i want in the plate, then fill all tiny 96 wells. like putting shells into the congkak board, times 9 or 10. i can already feel the excitement doing this =.=. .
2) for one extract(yes JUST ONE out of six) i gotta dilute them into somemore tiny tubes of 1.5ml. dilute dilute .
3) fill the 96 well all over again, but this time with the different dilutions, not like the boring same old filling of wells in 1)
4) wait one or two days for the effect of the extract on cells
5) put the magical white powder(which can cause mutation by the way) into the wells, wait. . . the dissolve it with something else and lastly
6) let a computer analyse the wells.

 oh wait, thats not all man, you wish . . .

7) repeat each plant twice and with different waiting times for extract to treat the cells.
8) repeat
9) repeat repeat
10) .  . am i dead yet. .
11) repeat. . .

 ah well maybe one day in the tissue culture lab i may finally finish it.  till then,fit just goes on and on an on. . . . (taiyo-dynamite)

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